The Microscopy Knowledge Portal
The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!
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Topics – A Vast Amount of Expert Knowledge
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Latest Articles
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Perform Microscopy Analysis for Pathology Ergonomically and Efficiently
The main performance features of a microscope which are critical for rapid, ergonomic, and precise microscopic analysis of pathology specimens are…
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Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on…
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Key Factors to Consider When Selecting a Stereo Microscope
Stereo microscopes are often nicknamed the “workhorse” of the lab or production site. Users spend many hours looking through the eyepieces inspecting,…
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Getting Sharper 3D Images of Thick Biological Specimens with Widefield Microscopy
Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of…
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How to Sanitize a Microscope
Due to the current coronavirus pandemic, there are a lot of questions regarding decontamination methods of microscopes for safe usage. This…
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What Does 30,000:1 Magnification Really Mean?
One important criterion concerning the performance of an optical microscope is magnification. This report will offer digital microscopy users helpful…
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The Fundamentals and History of Fluorescence and Quantum Dots
At some point in your research and science career, you will no doubt come across fluorescence microscopy. This ubiquitous technique has transformed…
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Eyepieces, Objectives and Optical Aberrations
For most microscope applications, there are generally only two sets of optics which are adjusted by the user, namely, the objectives and the…
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Koehler Illumination: A Brief History and a Practical Set Up in Five Easy Steps
The technique of Koehler Illumination is one of the most important and fundamental techniques in achieving optimum imaging in any given light…
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Immersion Objectives: Using Oil, Glycerol, or Water to Overcome some of the Limits of Resolution
To examine specimens at high magnifications using the microscope, there are a number of factors which need to be taken into consideration. These…
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Graphene-Based Microbots for Toxic Heavy Metal Removal and Recovery from Water
Heavy metal contamination in water is a serious risk to the public health and other life forms on earth. Current research in nanotechnology is…
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Collecting Light: The Importance of Numerical Aperture in Microscopy
Numerical aperture (abbreviated as ‘NA’) is an important consideration when trying to distinguish detail in a specimen viewed down the microscope. NA…
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Introduction to Widefield Microscopy
One of the most basic microscopy techniques is known as ‘Widefield Microscopy’. It is fundamentally any technique in which the entire specimen of…
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Optimization of the Interplay of Optical Components for Aberration free Microscopy
Optical microscopes are used to magnify objects which are otherwise invisible for the human eye. For this purpose high quality optics is necessary to…
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Milestones in Incident Light Fluorescence Microscopy
Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our…
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Video Talk by Kurt Thorn: The Abbe Diffraction Experiment
This lecture describes the famous experiments of Ernst Abbe which showed how diffraction of light by a specimen (and interference with the…
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Microscope Resolution: Concepts, Factors and Calculation
在显微镜中,术语“资源lution’ is used to describe the ability of a microscope to distinguish detail. In other words, this is the minimum…
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Image Gallery: Application Options with Leica DM2500 LED Microscope
The number of applications in microscopy can be as manifold as life itself. Different specimens demand different contrast methods to get a decent…